Laboratory Practicals of Microbiology (LRR / MIKCV)

Course objectives:

The goal of this course is to teach students basic microbiological techniques and to show them the importance of microorganisms in nature and in daily life.

Requirements on student

100% attendance on practice, write laboratory reports, obtain minimum 85% points in report evaluation and 60% points in control tests.

Content

Laboratory course consists of several thematic parts: 1. Safety in microbiological laboratory. 2. Preparation of cultivation media and sterile tools. 3. Cultivation and morphology of microorganisms. 4. Aseptic technique and methods for isolation of microorganisms. 5. Determination of the number of microorganisms. 6. Influence of various factors on microbial growth. 7. Physical and chemical methods of microbial growth control. 8. Microscopic examination of microorganisms. 9. Principles of bacterial identification. 10. Eukaryotic microorganisms. 11. Presence and analysis of microorganisms in water and soil. 12. Microorganisms in food.

Activities

Guarantors and lecturers

Guarantors: doc. RNDr. Jitka Frébortová, Ph.D.,
Tutorial lecturer: doc. RNDr. Jitka Frébortová, Ph.D., Mgr. Natsaran Saichana, Ph.D.,
Seminar lecturer: doc. RNDr. Jitka Frébortová, Ph.D.,

Literature

Recommended: Laboratorní cvičení z mikrobiologie. (Frébortová J.)
On-line library catalogues

Time requirements

Activities	Time requirements for activity [h]
Attendace	40
Homework for Teaching	15
Total	55

Prerequisites – other information about course preconditions

–

Competences acquired

Ability to apply fundamental microbiological techniques.

Teaching methods

Observation
Demonstration
Laboratory Work

Assessment methods

Student performance