Practicals in Cell Biology (LRR / BUBCV)

Course objectives:
To learn practical methods used in cell biology.
Requirements on student
Full participation at the practicals is required (maximum two absences may be tolerated for serious reasons and the experiments may be conducted in a different class as decided by the teacher); all experimental tasks must be completed, reports submited and approved for each task. The class is concluded with a final written test.
Content
Microscopy. Probing chemical composition of cell. Cell separation and cell cultures. Membranes, their compostion and characteristics. Cell transport. Osmotic features of cell. Animal cell. Plant cell. Cytochemical localization of cell components. Isolation of cells and cell counting. Cell viability assays. Cytotoxicity.
Activities
Guarantors and lecturers
Literature
  • Recommended: Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P. (1998). Základy buněčné biologie. Espero Publishing, Ústí nad Labem, 2000.
  • Recommended: Paleček, J. Biologie buňky, I. Základy mikroskopické cytologie. UK, Vydavatelství Karolinum, Praha, 1996.
  • Recommended: Habrová, V. Mikroskopická technika. SPN Praha, 1990.
  • Recommended: Razin S. E. Plant microtechnique and microscopy. Oxford University Press, Inc., 1999.
  • On-line library catalogues
Time requirements

Activities

Time requirements for activity [h]

Attendace

 24

Homework for Teaching

12
Total

36

Prerequisites – other information about course preconditions
To learn basic methods used in cell biology.
Competences acquired
Student should be able to (after attending the course): Microscopy. Probing chemical composition of cell. Cell separation and cell cultures. Membranes, their compostion and characteristics. Cell transport. Osmotic features of cell. Animal cell. Plant cell. Cytochemical localization of cell components. Isolation of cells and cell counting. Cell viability assays. Cytotoxicity.
Teaching methods
  • Laboratory Work
Assessment methods
  • Written exam
  • Analysis of Activities ( Technical works)
  • Systematic Observation of Student