Laboratory Training (LRR / LAB)

Course objectives:
The laboratory safety, chemicals-storage, purity, basic chemical calculations, common buffers preparation instructions.
Requirements on student
in extent of the lectures
Content
The laboratory safety, chemicals-storage, purity, basic chemical calculations, common buffers preparation instructions. Weighting and Scales: mechanical scales, digital scales, solid and liquid chemicals weighting. Volume and admeasurements determination: laboratory glass volume measurement, pipeting and various digital pipetes. Common laboratory equipment: thermostats, water baths, shakers etc. Electromigration techniques: agarose and polyarylamide gel electrophoresis, gel sekvenation, isoelectric focusation. Potentiometry: equipment calibration, buffers, pH-measurement, conductivity measurement, oximetry. Centrifugation and sedimentation: sedimentation, flotation, coagulation, filtration and microfiltration. Spectrophotometry: colorimetry, UV-VIS spectrometry, AAS, calibration curves. Drying and cooling: basic principles, solids drying, liquid and gas chemicals, cooling mixtures. Distillation: evaporization, normal and vacuum distillation. Chromatography: basic principles, distribution according to experimental arrangement and type of stacionary phase. Excursion in the laboratory of Molecular and Cell biology.
Activities
Guarantors and lecturers
Literature
  • Recommended: Ferenčík, M. a kol. Biochemické laboratórne metódy, ALFA, Bratislava 1989. Alfa Bratislava, 1981.
  • Recommended: Kašpárek, F. a kol. Cvičení z laboratorní techniky. Vydavatelství UP Olomouc, 2000.
  • Recommended: Peč, P. a kol. Laboratorní cvičení z biochemie. Vydavatelství UP Olomouc, 2000.
  • Recommended: Káš, J. a kol. Laboratorní cvičení z biochemie. Nakladatelství Olomouc, 2000.
  • Recommended: Pazdera, P. a Potáček, M. Laboratorní cvičení z organické chemie. Vydavatelství UJEP Brno, 1984.
  • Recommended: Mikeš, V. Základní biochemické praktikum. Vydavatelství MU Brno, 1992.
  • Recommended: Eysseltová, J. a kol. Základy laboratorní techniky. Nakladatelství Karolinium Praha, 2000.
  • On-line library catalogues
Prerequisites – other information about course preconditions
–
Competences acquired
Student should be able to (after attending the course): – work safely in the laboratory – prepare basic solutions and measure their physical characteristics – perform different Electromigration, Spectrophotometry and Chromatography techniques
Teaching methods
  • Laboratory Work
Assessment methods
  • Written exam